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Centrifugation

In this article we are going to study about Centrifugation and its application. At last we are going to discuss some important questions related to the topic.

Centrifugation is a separation technique that uses a centrifugal force to separate two liquid mixes. The denser component of the mixture flows away from the shaft in this operation, while the lighter component moves towards it. 

Since churning is excessively quick and less time-consuming, this method is most commonly utilised. The container carrying the mixture is rotated in the middle by a rotator in this method, allowing the components of the mixture to easily separate under the centrifugal force.

Churning

The combining of cream to make butter is referred to as the stirring process. Previously, this operation could only be completed through manual methods. 

For example, in a traditional frothier, vessel or sour cream was fitted with a piston that was rotated to agitate it. Another hand-operated device with to and fro movement set instead of rotation.

Methods of Separation

Many of the materials we come into contact with are mixtures. Two or more chemicals are blended in these combinations. In some cases, such a blend may not be possible to use in homes and businesses. 

Distinct qualities of different substances in a mixture include size, hardness, density, solubility, texture, weight, and so on. They are separated from a mixture by differences in physical properties.

Based on the type of mixture, different methods of separation are used. Below some are listed;

  • Filtration
  • Hand-picking
  • Threshing
  • Winnowing
  • Sieving
  • Sedimentation and decantation
  • Magnetic separation
  • Centrifugation
  • Evaporation
  • Distillation

Centrifugation

In simple terms, the centrifugation process separates skim milk from whole milk required to manufacture ghee and butter. Water and garments are separated using this method. After the washing and rinsing process is complete, washing machines employ this technology to spin the garments at a high speed to dry them.

Centrifugation is used in laboratories to separate cells from plasma in the blood. Centrifugation can be used to remove chalk dust from water. The wine is stabilised by centrifugation. It is possible to isolate particles from a high-velocity air stream. In various purification processes, the process is utilised to separate proteins.

It is a method of separating lighter from heavier substances by rapidly churning or rotating the mixture. Separating butter from cream is a typical process used at home. High-speed churning is used to make the cream. The butter particles split during churning and rise to the top of the liquid because they are lighter.

Principle of Centrifugation

  • The centrifuge uses the sedimentation concept.
  • The centrifugation technique works on the premise of separating particles suspended in liquid media using a centrifugal field. In the centrifuge, these are arranged in tubes or bottles in a rotor.
  • Sedimentation is the process through which suspended matter settles out of fluids due to gravity. The suspended material can be clay or powder particles. Tea leaves, for example, fall to the bottom of a teacup.
  • Particles larger than 5 micrometres are separated using a simple filtration procedure, whereas particles smaller than 5 micrometres do not settle due to gravity. To separate those particles, the central force is useful.

Application of Centrifugation

  • It is used to separate cream in dairies.
  • It is used in washing machines to squeeze water out of wet garments.

The separation procedure is determined by the nature of the mixture’s components. The following are the numerous physical methods often used to separate the components of mixtures comprising liquids, in addition to churning and centrifugation:

Fractional Distillation

Fractional distillation is the process of distilling two (or more) miscible liquids and collecting the distillate in fractions boiling at various temperatures. The more volatile (lower boiling) liquid is distilled first, then the less volatile distilled water (higher boiling).

A simple fractionation column is a glass bead-filled tube. The beads provide a surface for the vapours to cool and condense again.

Application of Fractional Distillation

  • To separate mixtures of miscible liquids, fractional distillation is used.
  • Used to separate crude oil into useful fractions like kerosene, gasoline, and diesel, among others.
  • Gases are separated from the air using this device.

Separation by Separating Funnel

A separating funnel can be used to separate two immiscible liquids.

The density difference between two immiscible liquids determines how well they can be separated with a separating funnel. The bottom layer is made up of the denser liquid, while the upper layer is made up of the lighter liquid.

This process can be used to separate water and kerosene, for example. The bottom layer of the funnel is heavier water, while the top layer contains lighter kerosene.

Conclusion

Centrifugation is a separation technique that uses a centrifugal force to separate two liquid mixes. The denser component of the mixture flows away from the shaft in this operation, while the lighter component moves towards it. 

Centrifugation is used in laboratories to separate cells from plasma in the blood. Centrifugation can be used to remove chalk dust from water. The wine is stabilised by centrifugation. It is possible to isolate particles from a high-velocity air stream. In various purification processes, the process is utilised to separate proteins.

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