Microscope is a piece of scientific equipment that magnifies microscopic items that can’t be seen with the naked eye. Furthermore, we can see numerous species, such as a cell, the structure of dust particles, and so on, using a microscope.
A simple microscope is a laboratory tool used in a variety of branches of study to see small objects that need to be examined in more detail. It permits researchers to look into objects and structures that aren’t apparent to the naked eye. It may be used to view things with a size between 0.4 and 0.7 µm.
Simple Microscopes
A simple microscope is a scientific instrument that magnifies small objects to make them visible to the naked eye, which would otherwise be invisible. It’s essentially a pair of powerful magnifying glasses with a short focal length and a double convex lens. The hand lens and reading lens are the most typical examples of this type of device. The magnification process creates a virtual image that cannot be presented on a screen like a real one.
Antoine Van Leeuwenhoek, a Dutch scientist, constructed the first simple microscope, which consisted of a small single high-powered converging lens for inspecting freshwater microorganisms. This was created mostly with a light microscope.
A compound microscope, which has two or more than two convex lenses, works on the same concept as a simple microscope. When more magnification/magnifying power is required, a compound microscope is utilised, which has two or more than two convex lenses.
When an object is put within a convex lens’ focal length, the image created is virtual, erect, and magnified, allowing observers to view it.
Parts of a Simple Microscope
Here are some Parts of Microscopes which are given below:
Eye-Piece
The eyepiece is the lens at the top of the microscope that is used to examine the samples. The magnification ranges from 10 times to 15 times.
Base
The base of the microscope provides support for the entire structure.
Tube
The eyepiece is connected to the objective lenses by a tube.
Objective Lens
The objective lenses are used to see the specimen/object precisely. These are color-coded, and magnifications of 10X, 40X, and 100X can be discovered. The lower power lenses are the shortest, while the higher power lenses are the longest.
Revolving nose-piece
The turret is another name for the revolving nose-piece. This is used to hold other objective lenses and can be rotated to meet the needs of the user when viewing any samples.
Diaphragm
The diaphragm is used to control the quantity of light that passes through the stage.
Specimen stage
This is the platform on which the slides with samples are placed.
Stage Clips
Stage clips are used to keep the slides in position and the sample steady while being viewed.
Coarse adjustment knob
It’s used to focus on scanning using the coarse adjustment knob.
Adjustment Knob
It’s used to focus on oil with the fine adjustment knob.
Arm
The arm connects and supports the tube to the microscope’s base.
Power Switch
The power switch is used to turn the microscope on and off.
Uses of Microscope
The microscope is used for a variety of applications in a variety of scientific fields. The microscope is an essential tool in forensic science as well as traditional medical science. These, on the other hand, are mostly utilised for:
Analysing Tissue
Histologists frequently use a microscope to examine cells and tissues. For example, histologists can use a microscope in combination with other measures to evaluate whether a slice of tissue is cancerous.
Studying Forensic Data
Evidence gathered at a crime scene may contain information that cannot be seen with the naked eye. Striations in bullets, for example, can be inspected under a microscope to discover if they match rounds fired from a specific rifle.
To keep a check on the Ecosystem
Field biologists frequently use microscopes to track the number and diversity of species in a certain place over time in order to assess the health of a particular ecosystem, such as a stream.
Exploring Cell Proteins
When studying the function of proteins within cells, researchers find microscopes to be an important tool. Many proteins can now be tagged and analysed in live cells because to advances in technology.
Examining the Structure of an atom
Scientists have used powerful microscopes like atomic force microscopes to study the surfaces of individual atoms.
Magnification of a Simple Microscope
The Magnifying formula of a Simple Microscope is given by:
M=1+DF
Here, D is the least distance and F is the focal length.
Conclusion
A microscope is an optical instrument for observing and studying small (microscopic) objects, such as cells. The picture of an object is enlarged in the microscope by at least one lens. This lens bends light toward the eye, giving the impression that an item is larger than it actually is. A microscope is made up of three parts: the head, the base and the arm. The observer’s eye is closer to the eyepiece lens.
In comparison, the objective lens, which magnifies the image by 400–100 times, is closer to the object. A modern microscope’s adjustment knobs, aperture, stage, nosepiece, condenser, and diaphragm are crucial components that help provide a highly magnified and resolved image of the object.