Biotechnology in Tissue Culture

When fragments of tissue from an animal or plant are transferred to an artificial environment where they can continue to survive and function, this is known as tissue culture. The cultured tissue may be made up of a single cell, a population of cells, or a whole or part of an organ, depending on the circumstances. A variety of cell behaviours can occur in cultured cells: cell proliferation; cell size; cell shape; cell function; specialised activity (muscle cells, for example, may contract); and interaction with other cells.

Cell and tissue culture are terms that are commonly used to refer to the cultivation of animal cells and tissues, with the more specific term plant tissue culture being used for the cultivation of plants. Montrose Thomas Burrows, an American pathologist, is credited with coining the term “tissue culture.”

Tissue Culture

Tissue culture is the in vitro aseptic culture of cells, tissues, organs, or a whole plant under controlled nutritional and environmental conditions, which is often used to produce plant clones. Tissue culture is also known as organ culture or organogenesis. The clones that are produced are true to type for the genotype that was chosen. It is under controlled conditions that the culture can grow and multiply, providing a favourable environment for their survival. These conditions include an adequate supply of nutrients, a pH medium that is appropriate for the environment, an appropriate temperature, and an appropriate gaseous and liquid environment.

Plant Tissue Culture

Plant tissue culture techniques, in addition to being used as a research tool, have recently gained significant industrial importance in the areas of plant propagation, disease elimination, plant improvement, and the production of secondary metabolites. Under controlled conditions, a single explant can be multiplied into several thousand plants in a relatively short period of time and space, regardless of the season or weather conditions, on a year-round basis, from a single source. Micropropagation has been used successfully to grow and conserve endangered, threatened, and rare species because of the high coefficient of multiplication and low requirements for the number of initial plants and available space in comparison to other methods.

Furthermore, plant tissue culture is considered to be the most efficient technology for crop improvement because it allows for the production of somaclonal and gametoclonal variations to be produced. With the advancement of micropropagation technology, there is a tremendous opportunity to produce plants of superior quality, isolate useful variants in well-adapted high yielding genotypes, and improve disease resistance and stress tolerance capacities. 

The Method of Growing New Plants Through the Use of Tissue Culture

• A little bit of plant tissue is removed from the growing point or tip of the plant and then put on top of a sterile jelly that contains nutrients and plant hormones. Hormones cause the cells in plant tissue to divide more quickly, which results in the production of a high number of cells that combine to form an unstructured mass of material known as a ‘callus.’
• After that, the callus is submerged in a second jelly that has plant hormones in it. These plant hormones urge the callus to form roots
• After that, the callus that now has roots is placed on top of a jelly that contains a number of hormones that encourage the creation of new shoots
• The callus, which already possesses roots and shoots, will eventually divide into a number of smaller plantlets. This approach produces a large number of micro plantlets from a relatively small number of starting plant cells or tissue
• The plantlets are then moved, either into pots or directly into the ground, where they will have the opportunity to mature into full-grown plants

Technical Aspects of Plant Tissue Culture and Molecular Biology are Utilised in Genetic Transformation Technology for the Following Purposes:

• Production of improved crop varieties
• Plants that are disease-free are being produced 
• Genetic transformation
• Secondary metabolite production is a process.
• The development of varieties that are resistant to salinity, drought, and heat stress.

Advantages of Tissue Culture

• A new plant can be developed from a plantlet in a relatively short period of time.
• It is only necessary to use a small amount of the initial plant tissue.
• It is more likely that the new plantlets and plants will not be infected with any viruses or diseases.
• The procedure is not affected by the changing of the seasons and can be carried out at any time of the year.
• To carry out the operation, a comparatively modest amount of room is all that is required (ten times the plants in one-tenth of the space).
• The method of tissue culture helps to supply the consumer market with new subspecies and varieties of products, which can be done on a greater scale.
• People who are interested in cultivating difficult plants, such as specific varieties of orchid, feel that the tissue culture approach is more successful than standard soil cultivation methods.

Disadvantages of Tissue Culture

• Tissue Culture can involve more labour and cost more money.
• Because of the specific environment in which they are produced, there is a possibility that the plants that have been propagated will have a lower resistance to illnesses.
• It is vital that the material be tested before to being cultured; failure to pick up on any anomalies could lead to the new plants being infected with a disease.
• There is no assurance that the tissue culture will be successful, despite the fact that the likelihood of success being high provided the appropriate procedures are adhered to. There is still a possibility that the process will set off a secondary metabolic chemical reaction, which could result in the growth of the new explants or cells being stifled or perhaps terminated entirely.

Conclusion

Plant tissue culture, as an emerging technology, has a significant impact on both agriculture and industry, as it provides plants that are required to meet the ever-increasing demand of the world population. Agricultural genetics has made significant contributions to the advancement of agricultural sciences in recent years, and they are now considered to be an essential tool in modern agricultural production.

At a rate that has never been seen before, biotechnology is being introduced into agricultural practices. Using tissue culture, it is possible to produce and propagate plant material that is genetically homogeneous and disease-free. In vitro culture of cells and tissues is a useful tool for inducing somaclonal variation in a variety of species. Induced genetic variability caused by tissue culture could be exploited as a source of variability for the development of new stable genotypes.

It has been encouraging to see the application of biotechnological approaches for in vitro regeneration, mass micropropagation techniques, and gene transfer studies in tree species. Growing mature and/or immature zygotic embryos in vitro is a technique used to recover plants produced by intergeneric crosses that do not produce fertile seeds. A number of improved crop varieties with high yield potential and pest resistance can be made possible through genetic engineering.