NDA » NDA Study Material » Physics » Knowing About the Electron Microscope

Knowing About the Electron Microscope

Electron microscope is a modern technique used for image magnification with the beam of electrons featured within it. It is used for illumination, scanning for inorganic and other biological species.

This is used as a powerful tool through which high-resolution electrons are accelerated to illuminate the object’s body. In the first era of the twentieth century, many scientists came up with a proposition that cathode rays have the potential to increase the resolution powers in conventional microscopes. Electrons having 600000 volts can be used in microscopes for getting more clear and specific scans and illumination of objects. This work has described the significance and details of electron microscopy. Scanning procedures through an electron microscopy has also been revealed in this work. Price of an electron microscope and features of this instrument are thoroughly discussed. It has become an important topic in science that helps in scanning microorganisms and other objects. 

Electron microscope

This is a scientific instrument that helps in scanning or illuminating an object to a high scale by using high-resolution power carried within the electrons. Through this instrument material, topography, composition and other physical features and chemical features are well analysed. Topography includes analysing the physical property of an object where hardness, reflectivity are examined. Crystallographic information of an object is also clearly analysed using this instrument. Eliminating the limitations of light microscopy, this instrument was introduced. In the early quarter of the twentieth century, scientists started saying that 0.02 resolution and 500x and 1000x magnification power is not significant enough to clearly analyse. Hence, the need for an electron microscope came and its magnification power is more than 100000 x. “Transmission Electron Microscope (TEM)” is the type of electron microscope to be introduced first and it was exactly featured like a light microscope except instead of using a beam of light, beams of electrons were used here. This microscope works in the form where a beam of electrons is used using a positive specimen of the electron channel. Through a magnetic lens, the electrons get affected and then interaction takes place within the specified sample. 

Scanning in Electron Microscope

Scanning in microscopes has become easy due to the introduction of electron microscopes using electron beams. Scanning type of electron microscope is another type of electron microscope and “scanning electron microscopy” is considered as a technique for surface visualisation and characterization. It is considered as a high-resolution power of electron microscopes and uses a raster scanning pattern. It is an ideal technique for understanding the structures of nanoparticles. This electroscopic process uses backscattered electrons, “field emitting guns”. These features have high resolution and image magnification power. Environmentally type of scanning microscopy is a new feature of this technique where samples like wood, fibre do not require a coating before the microscopy process takes place. Another facility is that electron guns remain below the standard vacuum in the instrument. These microscopes produce the electrons at the top level of the chamber and then pass through magnetic lenses for checking. Scan coils control the electron position and it allows the electron beams to scan above the sample area. 

Price of electron Microscope

Wide range of price levels exists for a wide array of these microscopes. The minimum level for this type of microscope is 1.3 million for the microscope having 0.3 mm3 resolution power. New types of electron microscope can range from 80000 dollars to more than 1 million dollars. Though, the price level depends on resolution, magnification power and various configuration levels. The three different types of microscopes also differ in price. Among the three types scanning microscope has the lowest range of price varying from 80000 dollars to 1000000 dollars. On the other hand, transmission and reflection types have a higher cost with a price value of more than 300000 and 400000 dollars respectively

Conclusion

This work includes a Meta description of electron microscopy. It has discussed the introduction, working style and other different features of electron microscope. Through the FAQs provided in this work some other aspects like types of microscope, reason for using small-sized samples, importance of liquid nitrogen are discussed. This work also includes the characteristics of electron microscopes and why it was in need in the time of early 1930s in exchange for the traditional light microscope. Price of electron microscope and scanning electron microscopy is highlighted in this work. Scanning electron microscopes are considered a type of electron microscope. 

faq

Frequently asked questions

Get answers to the most common queries related to the NDA Examination Preparation.

What are the different types of electron microscopes?

Ans. There are generally three types of microscope and those are transmission type, scanning type and reflection typ...Read full

Is there any necessity for keeping a vacuum in the transmission type electron microscope?

Ans. Yes, there is a necessity for keeping a vacuum in the first column of this type of microscope. This is included...Read full

Is electron microscopy a lengthy process and how long does it take for scanning?

Ans. In general one to two weeks is taken for scanning, embedding, sectioning...Read full

Is the sample size small or large used in electron microscopes?

Ans. The sample size must be small in electron microscopes and it should be within 1 mm...Read full

Why is the cryo scanning type of electron microscope significant nowadays?

Ans. This type of microscope is effective to scan wet specimens or those specimens which are sensitive to beams. In ...Read full

Is liquid nitrogen used in transmission microscopes and why?

Ans. Liquid nitrogen is used in these types of microscopes to prevent contamination that can occur due to the vacuum...Read full