Cultivation of Bacteria-Culture Media and Method

In batch culture, all nutrients are supplied at the start of the Cultivation, with no supplements made later in the bioprocess. No additional nutrients are decided to add during the whole bioprocess. Only regulated components such as gasses, acids, and bases shall be added. Having followed a batch culture phase, a balance regarding a single element is formed. Under these circumstances, the Continuous and batch culture phase starts where fresh culture medium is added in the same percentage that it is removed. These bioprocesses are known as continuous cultures. They are incredibly beneficial when an overabundance of nutrient content would affect the outcome in inhibition, such as toxic chemical build-up or excessive warming. This is how batch culture and continuous culture work. 

Batch culture

In a batch culture process, all of the substances are aggregated simultaneously and then undertake interaction without any additional external input. This culture goes through three stages: the lag phase, wherein the microbes make adjustments to their surroundings; the exponential phase, in which the cells contribute to growth; and the stationary phase, in which all of the nutrient content has already been absorbed and the cells fade away. It is used in the manufacturing of wines and bread. 

Continuous culture

Continuous culture is a method in which components are added as required throughout the process, and the product is eliminated as they are established. Because of the constant availability of nutrients, the incredibly rapid phase is generally lengthened in this type. 

Continuous and batch cultures are a collection of practices for quantitatively nurturing microbes at moderate intensity growth rates under different growth constrictions so that the culture conditions continue to stay constant. 

Differences Between Batch And Continuous Fermentation 

The differences between batch and continuous fermentation are as follows:

1. Based on Fermentation Method:

• Batch fermentation: It is a limited batch.
• Continuous fermentation: It’s a new batch.

2. Nutrient supplementation

• Batch fermentation: In the start, all of the nutrients are introduced at once.
• Continuous fermentation: Throughout the batch, nutrients are essential.
• The rate of turnover

3. Batch fermentation: 

• Batch fermentation: The product formation turnover rate is low.
• Continuous fermentation: The product formation turnover rate is exceptionally high.

4. Product classification

• Batch fermentation: It is employed to extract secondary metabolites such as antibacterial agents.
• Continuous fermentation: It is employed to extract primary metabolites such as amino acids and proteins.

5. Conditions for growth Inside the fermenter

• Batch fermentation: The control parameters can be altered by adjusting the pH and dispersed oxygen levels.
• Continuous fermentation: The control parameters must be retained all through the batch’s operating condition.

6. Contamination possibilities

• Batch fermentation: It is less prone to contamination.
• Continuous fermentation: It is highly likely to be contaminated.

7. Consumption of nutrients 

• Batch fermentation: Nutrients are consumed more slowly.
• Continuous fermentation: Nutrients are rapidly depleted.

8. Microbiological stages

• Batch fermentation: Microbes progress through three stages: lag, exponential, and stationary.
• Continuous fermentation: The microbes go through a short lag phase before surviving in the exponential growth phase. 

Fermentation Methodology

The fermentation process happens in a container known as a fermenter or bioreactor. The fermentor’s design and nature vary according to the type of fermentation conducted. All fermentors can assess some of the fermentation variables such as temperature, force, pH, elapsed fermentation time, fluid pressure, density, and so on.

Fermentation Processes

There are three types of fermentation processes, namely: 

• fermentation in batches – Batch fermentation
• Feb-batch fermentation
• Ongoing culture – Continuous culture fermentation 

Batch and continuous fermentation are two industrial applications utilised in fermentation procedures for producing valuable microbe products. Batch fermentation is carried out in batches. Continuous fermentation occurs at periodic intervals while feeding nutrients and extract products. 

A fed-batch reactor improves the microenvironment of microbes by adding small amounts of ingredients at specific times. The fed-batch reactor lengthens the culture lag phase. 

Conclusion 

We discussed Cultivation of viruses- methods, methods of Cultivation of viruses, and other related topics through the study material notes on Cultivation of viruses- methods. We are also about isolation purification and the Cultivation of viruses to give you proper knowledge.  

 Fermentation is the metabolic pathway by which natural components are separated in the lack of oxygen with the assistance of microbes. It’s often used in manufacturing applications to make liquors and food ingredients. Fermentation can be conducted in four various ways: batch fed-batch, continuous, and open fermentation.