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Transformation Efficiency

Lesson 16 of 16 • 24 upvotes • 9:01mins

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Virendra Singh

Transformation efficiency is the efficiency by which cells can take up extracellular DNA and express genes encoded by it. This is based on the competence of the cells. It can be calculated by dividing the number of successful transformants by the amount of DNA used during a transformation procedure

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1

Course Overview

3:53mins

2

Mole Concept

8:17mins

3

Mole Concept - Examples from DBT JRF

8:50mins

4

Ionic Strength

8:48mins

5

PH and Buffer Concept

8:05mins

6

Reaction Kinetics

9:03mins

7

Bioenergetics

11:08mins

8

Enzyme: Activity and Specific activity

10:18mins

9

Enzyme: Fold purification and Recovery

10:23mins

10

Enzyme kinetics

8:15mins

11

Enzyme kinetics: Numerical Problems

9:01mins

12

Beer Lambert's Law and Partition Coefficient

9:24mins

13

Problems on DNA

9:33mins

14

Cell Growth and Kinetics

8:30mins

15

Restriction Mapping

9:16mins

16

Transformation Efficiency

9:01mins

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