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Restriction Mapping

Lesson 15 of 16 • 29 upvotes • 9:16mins

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Virendra Singh

A restriction map is a map of known restriction sites within a sequence of DNA. Restriction mapping requires the use of restriction enzymes. In molecular biology, restriction maps are used as a reference to engineer plasmids or other relatively short pieces of DNA, and sometimes for longer genomic DNA

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1

Course Overview

3:53mins

2

Mole Concept

8:17mins

3

Mole Concept - Examples from DBT JRF

8:50mins

4

Ionic Strength

8:48mins

5

PH and Buffer Concept

8:05mins

6

Reaction Kinetics

9:03mins

7

Bioenergetics

11:08mins

8

Enzyme: Activity and Specific activity

10:18mins

9

Enzyme: Fold purification and Recovery

10:23mins

10

Enzyme kinetics

8:15mins

11

Enzyme kinetics: Numerical Problems

9:01mins

12

Beer Lambert's Law and Partition Coefficient

9:24mins

13

Problems on DNA

9:33mins

14

Cell Growth and Kinetics

8:30mins

15

Restriction Mapping

9:16mins

16

Transformation Efficiency

9:01mins

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