RACE and RAPD (in Hindi)

Lesson 7 of 13 • 112 upvotes • 9:50mins

Virendra Singh

RAPD stands for 'Random Amplification of Polymorphic DNA'. It is a type of PCR, but the segments of DNA that are amplified are random. The scientist performing RAPD creates several arbitrary, short primers, then proceeds with the PCR using a large template of genomic DNA, hoping that fragments will amplify. RACE can provide the sequence of an RNA transcript from a small known sequence within the transcript to the 5' end (5' RACE-PCR) or 3' end (3' RACE-PCR) of the RNA. This technique is sometimes called one-sided PCR or anchored PCR.