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Real Time PCR (in Hindi)
Lesson 6 of 13 • 78 upvotes • 8:29mins
Virendra Singh
Real-time PCR (RT-PCR) is also called quantitative PCR or qPCR. The key feature in RT-PCR is that amplification of DNA is detected in real time as PCR is in progress by the use of fluorescent reporter. The fluorescent reporter signal strength is directly proportional to the number of amplified DNA molecules.
(Hindi) Techniques in Molecular Biology : CSIR-UGC NET
13 lessons • 1h 49m
1
Course Overview (in Hindi)
3:16mins
2
Polymerase Chain Reaction (PCR) (in Hindi)
8:42mins
3
PCR: Primer Design and DNA Polymerase (in Hindi)
10:19mins
4
PCR :Cycle (in Hindi)
9:33mins
5
PCR: RT, Nested, Inverse and Anchored PCR (in Hindi)
9:12mins
6
Real Time PCR (in Hindi)
8:29mins
7
RACE and RAPD (in Hindi)
9:50mins
8
RFLP and AFLP (in Hindi)
8:43mins
9
SSR: Simple Sequence Repeats (in Hindi)
8:35mins
10
FISH (Fluorescence in- situ hybridization)
8:01mins
11
DNA sequencing: Sanger method
8:44mins
12
DNA sequencing: Maxam Gilbert method
8:12mins
13
Automated sequencing
8:04mins
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