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DNA Fingerprinting (in Hindi)
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DNA fingerprinting is explained in this lesson.

Hariom Patel
MBBS student at AIIMS, New Delhi (AIR 38-AIIMS 2016)

U
Unacademy user
please cover convertibility of rupee and partial convertibility of rupee mam please it helps lot
Surabhi Agrawal
2 years ago
next course would be on the same topic
  1. DNA FINGERPRINTING By HARIOM PATEL


  2. e 99.9 per cent of base sequence among humans is the same human genome as 3 109 bp Difference? sequencing the DNA every time would be a daunting and expensive task


  3. repetitive DNA-differences Major peak- bulk DNA satellite DNA


  4. base composition (A T rich or G:C rich), o length of segment, 'number of repetitive units, Divide Satellite DNA into-Mini,micro are non coding These sequence show high degree of polymorphism and form the basis of DNA fingerprinting Polymorphism (variation at genetic level) arises due to mutations


  5. Allelic sequence variation has traditionally been described as a DNA polymorphism if more than one variant (allele) at a locus occurs in human population with a frequency greater than 0.01. If an inheritable mutation is observed in a population at high frequency, it is referred to as DNA polymorphism


  6. DNA Fingerprinting was developed by Alec Jeffreys In molecular biology, a hybridization probe is a fragment of DNA or RNA of variable length (usually 100-1000 bases long) which can be radioactively labeled. It can then be used in DNA or RNA samples to detect the presence of nucleotide sequences (the DNA target) that are complementary to the sequence in the probe


  7. 3 DNA is cut into fragments by a 2. DNA is extracted from blood cellsrestriction encyme 4. The DNA fragents are separated into bunds during electrophoresis n an agarose gcl I. Blood sample II. The X-tay fil . The DN.A band pattern in the gel is = i, developed 10 make visible the transferrod to a nylon membrane by a techinque known as Southern blotting pattern of bands which is known as a DNA fingerprint I- PROCESS 6. The radioactive DNA probe is prepared 10. X-ray film is placed next to the membrane to detect the radioactive patter 7.The DNA probe binds to specific DNA sequences on the membrane 8. Excess DNA probe is washed off 9. At this stage, the radiouctivity probe is bound to the DNA patiern on the membrane Steps in DNA fingerprinting techinque