BIOTECHNOLOGICAL APPLICATIONS IN MEDICINE By HARIOM PATEL
Genetically Engineered Insulin
In 1983, Eli Lilly an American company prepared two DNA sequences corresponding to A and B, chains of human insulin and introduced them in plasmids of E. coli to produce insulin chains.
Gene Therapy adenosine deaminase (ADA) defic .Deletion of the gene for adenosine deaminase Cured by- bone marrow transplantation enzyme replacement therapy
e lymphocytes from the blood of the patient are grown in a culture outside the body. A functional ADA cDNA (using a retroviral vector) is then introduced into these lymphocytes, which are subsequently returned to the patient. . Cells dice . Repeated infusions e If done in embryonic stages ?
Molecular Diagnosis . Recombinant DNA technology, e Polymerase Chain Reaction (PCR) e Enzyme Linked Immuno-sorbent Assay (ELISA)