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Introduction to Metabolism
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Will deal with types of metabolic reactions, methods to study metabolism and notes on marker enzymes!!

S Krittika
M.Sc Advanced Biochemistry- University of Madras.Awaiting INSPIRE fellowship. Believe in sharing is caring,as knowledge is not materialistic

Unacademy user
it was more like rewind what I had studied before. Nice session.. Do well
  1. Course 2- Lesson 1- Introduction to metabolism Presented by S.Krittika

  2. Metabolism o Metabolism - the chemical reactions in a cell: Two categories of cellular chemical reactions: Anabolic Reactions oBuild larger molecules for growth, repair, reproduction Dehydration Synthesis Reactions orequire energy and nutrients Catabolic Reactions Breakdown larger molecules Hydrolysis Reactions mobilize nutrients for energy making it available to the cell

  3. Metabolism o Metabolism is the sum of all anabolic and catabolic reactions that occur in the cell The metabolism of cells is carried out and controlled by the enzymes There are catabolic enzymes - those that cleave larger molecules into smaller ones Eg: Hydrolysis Reactions There are also anabolic enzymes - those that assemble smaller molecules into larger ones Eg: Dehydration Reactions

  4. Catabolic reactions The most common set of catabolic reactions in animals can be studied under 3 stages: 1) Conversion of complex molecules into their building blocks 2) Formation of simple intermediates 3) Final oxidation of Acetyl-CoA

  5. Anabolic reactions Anabolism involves 3 different steps: 1) The production of precursors 2) Activation into reactive forms 3) Assembly of the precursor forms Organisms differ in, substances they use to construct their molecules. (Autotrophs and heterotrophs).

  6. Methods to study metabolisnm 1) Whole organism study: - Radioisotopes employed in TRACER technique Common Radioisotopes are isotopes of For studies in carbohydrate metbolism In cholesterol biosynthesis For studying protein metabolism - -

  7. 2) Isolated or perfused organ studies 3) Tissue slice studies 4) Whole cells- using FACS, trypsin or collagenase 5) Cell free systems- shear force and differential centrifugation. 6) Purified components

  8. Marker enzymes An enzyme molecule or a bio-chemical compound that is almost extensively present in one particular organelle. USES: 1) Cell biomarkers used to characterize a cell type 2) In isolation of target cellular components 9