MSc Biotechnology || educator || reader || foodie || exploring science and research || GATE BT ||

Anagha Pisal

The course is about Introduction, tools in rDNA technology, various cloning strategies and applications.

English

Recombinant DNA technology: GATE

The lesson is about the general idea behind the recombinant DNA technology and what mechanism it's involves with applications

Introduction to recombinant DNA Technology

The lesson deals with the enzymes involved in DNA MANIPULATION and a general introduction and few terms related

Enzymes in DNA Manipulation - Part 1

The lesson deals with the enzymes, restriction endonuclease and and DNA LIGASE

Enzymes in DNA manipulation - 2

The lesson deals with the enzyme -  alkaline phosphatase, terminal deoxynucleotidyl transferase, polynucleotide kinase

Enzymes in DNA Manipulation - Part 3

The lessons deals the enzyme reverse transcriptase and different polymerases along this RNase H and DNase l

Enzymes in DNA Manipulation - Part 4

A short explanation about the role of Linkers and Adaptors in the formation for sticky ends.

Linkers and Adaptors

The lesson deals with the characteristics of vectors and all about the plasmids as cloning vector

Cloning vectors

The lesson describes the different examples of plasmid vectors.

Plasmid vectors

The lesson deals with the lambda phage as the vector for rDNA technology

Vectors : Lambda phage vector

The lessons deals with the phage M13 as a vector system.

Phage M13 vector

The lesson deals with the details about the hybrid vector i.e. phagemid

Phagemid vector

The lessons deals with the hybrid vector system i.e. cosmids

Cosmid vector

The lesson deals with the idea about the fosmid vectors

Fosmid vectors

The lesson deals with the yeast artificial chromosome as vector system

Yeast Artificial Chromosome vectors

The lesson deals with the Bacterial Artificial Chromosome as a cloning vector system

Bacterial Artificial Chromosome

The lesson deals with the direct selection method for recombinants

Screening of recombinants : direct selection method

The lesson deals with the blue white screening method for selection of recombinants

Blue - White screening

The lesson deals with the insertional inactivation method for selection of recombinants

Insertional inactivation

The lesson deals with the genomic library and it's construction

Genomic Library

The lesson deals with the construction of cDNA LIBRARY

CDNA library

The lesson deals with the isolated together DNA from the bacterial cells and chemical and methods involved

Lessons

28 lessons • 3h 34m

Invalid Date

NaN

Introduction to recombinant DNA Technology

Invalid Date

NaN

Enzymes in DNA Manipulation - Part 1

Invalid Date

NaN

Enzymes in DNA manipulation - 2

Invalid Date

NaN

Enzymes in DNA Manipulation - Part 3

Invalid Date

NaN

Enzymes in DNA Manipulation - Part 4

Invalid Date

NaN

Linkers and Adaptors

Invalid Date

NaN

Cloning vectors

Invalid Date

NaN

Plasmid vectors

Invalid Date

NaN

Vectors : Lambda phage vector

Invalid Date

NaN

Phage M13 vector

+ See all lessons