My name is Bhagyashree.I have pursued masters in Biotechnology from Bangalore university. Admin of youtube channel  bhagya's biology.I love 

Bhagyashree Choudhary

The course covers the different lessons of plant tissue culture such as,somatic hybridization,embryo culture,germplasm and various other topics.It also covers the applications of plant tissue culture.

English

Plant Tissue Culture

This lesson covers the overview of the course that what is the objective of the course where the basics of plant tissue culture,applications of plant tissue culture and the techniques involved in the plant tissue culture will be covered.It also tells about the targeted audience and the topics that will be covered in the course.

Overview

This video covers the basics of plant tissue culture such as benefits of plant tissue culture,structure of plant,terms used in plant tissue culture,history of plant tissue culture

Basics of Plant Tissue Culture - 1

This lesson explains the basics of plant tissue culture that is the type of culture depending on explant such as protoplast,cell,callus,organ culture and the basic procedure used in plant tissue culture.It also covers callus,suspension culture in detail and the applications of callus culture.

Basics of Plant Tissue Culture - 2

This lesson explains the cell culture and it's applications and the technique of cell culture and also the types of culture that is the continuous and batch culture

Basics of Plant Tissue Culture - 3

This lesson covers the basics of plant tissue culture synchronization of suspension culture,methods used for synchronization of suspension culture- physical and chemical methods,methods used for isolation of single cells-Bergman plating technique,microdrop method,microchamber method,raft tissue nurse technique,applications of plant tissue culture are covered

Basics of Plant Tissue Culture - 4

This lesson covers the secondary metabolites ,advantages and disadvantages of the secondary metabolites and the applications of the secondary metabolites and the methods for selection of the cell lines,chemical and visual analysis and also the cell aggregrate cloning,cell cloning

Secondary Metabolites in Plant Cultures - 1

This lesson explains the secondary metabolites in plant cultures and explains the entrapment,methods of entrapment,selection for resistance,large scale cultivation of plant cells,free-cell suspension,immobilized cell culture,bioreactor for use of immobilized cells,two-phase system culture,hairy root culture,medium composition and effect of nutrients,effect of carbon,effect of nitrogen source

Secondary Metabolites in Plant Cultures - 2

This lesson explains the secondary metabolites in plant culture that covers the effect of phosphate,effect of plant growth regulator,precursor,effect of precursor,elicitor-induced production of secondary metabolites,phytoalexins,interaction for elicitor formation,methodology of elucidation,mechanism of action of elicitor, effect of environmental factors,biotransformation using plant call culture,secondary metabolite release and analysis

Secondary Metabolites in Plant Cultures - 3

This lesson explains the plant tissue culture media about the media,composition of media,major types of media,expression of concentration in media,constituents of media

Plant Tissue Culture Media - 1

This lesson explains the elements and their functions,growth regulators,the 4 growth regulator such as abscisic acid,aux in,cytokinin,and giberrelin and their functions,sterilization of media,preparation of media,selection of media

Plant Tissue Culture Media - 2

This lesson explains the introduction to somatic hybridization and protoplast culture and also the methods for isolation of protoplast and sources of protoplast,It also covers the basics of protoplast culture

Protoplast Culture and Somatic Hybridization - 1

This lesson explains the cybrids,hybrids,cybridization,methodology of cybridization,application of cybrids,somatic incompatibility concept

Cybrids (in Hindi)

This lesson explains the agar culture,nutrient components,liquid medium,method of protoplast culture such as feeder layer technique,microdrop method

Protoplast culture and somatic hybridization part 2

This video explains the regeneration of protoplast that is cell wall formation and development of callus

Lessons

14 lessons • 2h 12m

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Overview

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Basics of Plant Tissue Culture - 1

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Basics of Plant Tissue Culture - 2

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Basics of Plant Tissue Culture - 3

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Basics of Plant Tissue Culture - 4

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Secondary Metabolites in Plant Cultures - 1

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Secondary Metabolites in Plant Cultures - 2

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Secondary Metabolites in Plant Cultures - 3

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Plant Tissue Culture Media - 1

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Plant Tissue Culture Media - 2

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